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Catalog Number Size Price
LS-C668250-50 50 µl (1 mg/ml) $286 
LS-C668250-100 100 µl (1 mg/ml) $321 
PA2G4 / EBP1 Antibody
PA2G4 / EBP1 Antibody
PA2G4 / EBP1 Antibody
PA2G4 / EBP1 Antibody
PA2G4 / EBP1 Antibody
PA2G4 / EBP1 Antibody
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Polyclonal Rabbit anti‑Human PA2G4 / EBP1 Antibody (IHC, IF, WB) LS‑C668250

Polyclonal Rabbit anti‑Human PA2G4 / EBP1 Antibody (IHC, IF, WB) LS‑C668250

Antibody:
PA2G4 / EBP1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C668250-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PA2G4 / EBP1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EBP1 antibody LS-C668250 is an unconjugated rabbit polyclonal antibody to human EBP1 (PA2G4). Validated for ICC, IF, IHC, IP and WB.
Target
Human PA2G4 / EBP1
Synonyms
PA2G4 | ErbB-3 binding protein 1 | EBP1 | ErbB3-binding protein 1 | ErbB3-binding protein Ebp1 | p38-2G4 | HG4-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Immunogen
Recombinant full length protein of human EBP1
Specificity
Recognizes endogenous levels of EBP1 protein.
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin
  • ICC
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western blot analysis of EBP1 expression in Hela (A), K562 (B), PC12 (C), NIH3T3 (D), mouse liver (E) whole cell lysates. Immunohistochemical analysis of EBP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of EBP1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PA2G4 / EBP1
Q9UQ80 NM_006191 NP_006182.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/25/2024