Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > p56lck / LCK > LS-C392593
 
Print Page  
Catalog Number Size Price
LS-C392593-200 200 µl $393 
p56lck / LCK Antibody - Antibody staining LCK in human thymus sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
p56lck / LCK Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
p56lck / LCK Antibody - Anti-LCK Antibody at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
p56lck / LCK Antibody - Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
p56lck / LCK Antibody - Antibody staining LCK in human thymus sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
p56lck / LCK Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
p56lck / LCK Antibody - Anti-LCK Antibody at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
p56lck / LCK Antibody - Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
1 of 4
2 of 4
3 of 4
4 of 4

Monoclonal Mouse anti‑Human p56lck / LCK Antibody (clone 1526CT823.75.16, IHC, IF, WB) LS‑C392593

Monoclonal Mouse anti‑Human p56lck / LCK Antibody (clone 1526CT823.75.16, IHC, IF, WB) LS‑C392593

Submit Review LSBio Guarantee
Antibody:
p56lck / LCK Mouse anti-Human Monoclonal (1526CT823.75.16) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392593-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
p56lck / LCK Mouse anti-Human Monoclonal (1526CT823.75.16) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LCK antibody LS-C392593 is an unconjugated mouse monoclonal antibody to human LCK (p56lck). Validated for Flow, IF, IHC, Peptide-ELISA and WB.
Target
Human p56lck / LCK
Synonyms
LCK | Lck kinase | LSK | p56-LCK | Pp58lck | Tyrosine-protein kinase Lck | p56lck | Protein YT16 | Proto-oncogene Lck | YT16
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1526CT823.75.16
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About p56lck / LCK
P06239 NM_005356 NP_005347.3

Publications (0)

Submit a Publication for LS-C392593

Customer Reviews (0)

Submit a review for LS-C392593

Featured Products

p56lck / LCK Antibody - Anti-LCK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
IHC‑plus™ p56lck / LCK Antibody (clone Lck‑01) LS‑B1670
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
p56lck / LCK Antibody - Western blot analysis of extracts from JK cells.
p56lck / LCK Antibody (phospho‑Tyr394) LS‑C335849
Species: Human
Applications: Western blot
p56lck / LCK Antibody - Anti-LCK antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ p56lck / LCK Antibody (clone 33D196) LS‑B2049
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
p56lck / LCK Antibody - Anti-p56lck / LCK antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ p56lck / LCK Antibody LS‑B8908
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
p56lck / LCK Antibody - Western blot analysis of lysates from Jurkat cells, using Lck (Phospho-Tyr192) Antibody. The lane on the right is blocked with the phospho peptide.
p56lck / LCK Antibody (phospho‑Tyr192) LS‑C199194
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
p56lck / LCK Antibody - Immunohistochemical analysis of LCK staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
p56lck / LCK Antibody (Internal) LS‑C413134
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all p56lck / LCK Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com