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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C744987-25 25 µl (1.4 mg/ml) $235 
p35 Antibody - Western blot showing detection of 0.1 µg of recombinant p35 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein (arrow; expected MW: 69.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
p35 Antibody - Western Blot of rabbit anti-p35 antibody. Lane 1: p35 recombinant protein 50 ng load. Lane 2: p35 recombinant protein 100 ng load. Lane 3: p35 recombinant protein 250 ng load. Lane 4: p35 recombinant protein 500 ng load. Primary antibody: p35 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for p35. Other band(s): p35 splice variants and isoforms.
p35 Antibody - Western blot showing detection of 0.1 µg of recombinant p35 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein (arrow; expected MW: 69.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
p35 Antibody - Western Blot of rabbit anti-p35 antibody. Lane 1: p35 recombinant protein 50 ng load. Lane 2: p35 recombinant protein 100 ng load. Lane 3: p35 recombinant protein 250 ng load. Lane 4: p35 recombinant protein 500 ng load. Primary antibody: p35 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for p35. Other band(s): p35 splice variants and isoforms.
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Polyclonal Rabbit anti‑Borrelia burgdorferi p35 Antibody (Liquid, WB) LS‑C744987

Polyclonal Rabbit anti‑Borrelia burgdorferi p35 Antibody (Liquid, WB) LS‑C744987

Antibody:
Rabbit Polyclonal to Borrelia burgdorferi p35
Application:
WB, ELISA
Reactivity:
Borrelia burgdorferi
Format:
Unconjugated, Liquid
Price
Catalog Number
$235 
LS-C744987-25
25 µl
1.4 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Borrelia burgdorferi p35
Application:
WB, ELISA
Reactivity:
Borrelia burgdorferi
Format:
Unconjugated, Liquid

Specifications

Description
P35 antibody LS-C744987 is an unconjugated rabbit polyclonal antibody to borrelia burgdorferi p35. Validated for ELISA and WB.
Target
Borrelia burgdorferi p35
Host
Rabbit
Reactivity
Borrelia burgdorferi (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Liquid
Applications
  • Western blot (1:1000)
  • ELISA (1:5000)
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.
Specificity
This antibody is specific for Borrelia burgdorferi p35 protein. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 8/6/2020