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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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P2RY12 / P2Y12 Antibody (clone 1C2A9) LS‑C682595
P2Y12 antibody LS-C682595 is an unconjugated mouse monoclonal antibody to human P2Y12 (P2RY12). Validated for ELISA, Flow and WB.
Catalog
Size
Price
LS-C682595-100
100 µl
$365
Description
P2Y12 antibody LS-C682595 is an unconjugated mouse monoclonal antibody to human P2Y12 (P2RY12). Validated for ELISA, Flow and WB.
Target
Human P2RY12 / P2Y12
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1C2A9]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Immunogen
P2RY12 / P2Y12 antibody was raised against purified recombinant fragment of human P2RY12 (AA: extra mix) expressed in E. Coli.
Usage
Predicted Western blot MW: 39.4kDa
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
About P2RY12 / P2Y12
Q9H244 NM_022788 NP_073625.1

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Images

Western blot

Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.
Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)
Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot

Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.
Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)
Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot

Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.
Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)
Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot

Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.
Western blot analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.

Western blot

Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)
Western blot analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)

Flow Cytometry

Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).

Enzyme-Linked Immunosorbent Assay

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Requested From: United States
Date Requested: 12/16/2018
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