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Antibodies > p14ARF / CDKN2A > LS-C800349
 
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Catalog Number Size Price
LS-C800349-100 100 µl (1 mg/ml) $379 
LS-C800349-200 200 µl (1 mg/ml) $421 
p14ARF / CDKN2A Antibody - 1:200 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
p14ARF / CDKN2A Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
p14ARF / CDKN2A Antibody - Western blot analysis on HeLa cell lysates using p14 ARF antibody. The lane on the left is treated with the antigen-specific peptide.
p14ARF / CDKN2A Antibody - 1:200 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
p14ARF / CDKN2A Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
p14ARF / CDKN2A Antibody - Western blot analysis on HeLa cell lysates using p14 ARF antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human p14ARF / CDKN2A Antibody (IHC, IF, WB) LS‑C800349

Polyclonal Rabbit anti‑Human p14ARF / CDKN2A Antibody (IHC, IF, WB) LS‑C800349

Submit Review LSBio Guarantee
Antibody:
p14ARF / CDKN2A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800349-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
p14ARF / CDKN2A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CDKN2A antibody LS-C800349 is an unconjugated rabbit polyclonal antibody to human CDKN2A (p14ARF). Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human p14ARF / CDKN2A
Synonyms
p14 | p14ARF | CDKN2 | CDKN2A | MLM | p14 arf | ARF tumor suppressor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human p14 ARF.
Specificity
P14 ARF antibody detects endogenous levels of total p14 ARF.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Submit a Publication for LS-C800349

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Featured Products

p14ARF / CDKN2A Antibody - MLM Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human pancreas tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
p14ARF / CDKN2A Antibody (aa72‑101) LS‑C164218
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
p14ARF / CDKN2A Antibody - Detection of Human p14ARF by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-p14ARF used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
p14ARF / CDKN2A Antibody (aa125‑C‑term) LS‑C287247
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Product
p14ARF / CDKN2A Antibody (aa72‑101, APC) LS‑C207247
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
p14ARF / CDKN2A Antibody - Immunohistochemical analysis of p14 ARF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
p14ARF / CDKN2A Antibody (Internal) LS‑C351999
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
p14ARF / CDKN2A Antibody - IHC of p14 ARF on an FFPE Anal Carcinoma Tissue
p14ARF / CDKN2A Antibody (clone RBT‑p14) LS‑C763400
Species: Human
Applications: IHC - Paraffin, IHC - Frozen
RIPK1 / RIP Antibody - IHC of RIP in formalin-fixed, paraffin-embedded normal human tonsil using RIPK1 / RIP Antibody at 1:2000. A, low magnification over view of a secondary follicle. A1 and A2, successively higher magnifications of the germinal center from A. Hematoxylin-Eos in counterstain.
RIPK1 / RIP Antibody (aa269‑287) LS‑C147991
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
See all p14ARF / CDKN2A Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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