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Catalog Number Size Price
LS-C390797-100 100 µg (1 mg/ml) $574 
NUMA1 / NUMA Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with NuMA antibody (SPM300).  This image was taken for the unmodified form of this product. Other forms have not been tested.
NUMA1 / NUMA Antibody - Western blot testing of MOLT-4 cell lysate (nuclear fraction) with NuMA antibody (clone SPM300). This image was taken for the unmodified form of this product. Other forms have not been tested.
NUMA1 / NUMA Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with NuMA antibody (SPM300).  This image was taken for the unmodified form of this product. Other forms have not been tested.
NUMA1 / NUMA Antibody - Western blot testing of MOLT-4 cell lysate (nuclear fraction) with NuMA antibody (clone SPM300). This image was taken for the unmodified form of this product. Other forms have not been tested.
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Monoclonal Mouse anti‑Human NUMA1 / NUMA Antibody (clone SPM300, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390797

Monoclonal Mouse anti‑Human NUMA1 / NUMA Antibody (clone SPM300, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390797

Antibody:
NUMA1 / NUMA Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM300) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Price
Catalog Number
$574
LS-C390797-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NUMA1 / NUMA Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM300) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:

Specifications

Description
NUMA antibody LS-C390797 is an unconjugated mouse monoclonal antibody to human NUMA (NUMA1). Validated for Flow, IF, IHC and WB.
Target
Human NUMA1 / NUMA
Synonyms
NUMA1 | Structural nuclear protein | NuMA protein | SP-H antigen | NUMA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgM,k Monoclonal
Clone
SPM300
Conjugations
Unconjugated
Purification
PEG purified
Modifications
Azide-free, BSA-free. Also available Unmodified.
Immunogen
Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.
Specificity
Human NUMA1 / NUMA
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Western blot
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia. Optimal dilution of the NuMA antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
1X PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NUMA1 / NUMA
Q14980 NM_006185 NP_006176.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/5/2024