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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C391466-20 20 µg (0.2 mg/ml) $295 
LS-C391466-100 100 µg (0.2 mg/ml) $465 
Antibody

Nuclear Membrane Antibody (clone AE‑5) LS‑C391466

Nuclear Membrane Antibody (clone AE‑5) LS‑C391466

Antibody:
Mouse Monoclonal to Human Nuclear Membrane
Application:
ICC, IF
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$295 
LS-C391466-20
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Nuclear Membrane
Application:
ICC, IF
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Nuclear Membrane antibody LS-C391466 is an unconjugated mouse monoclonal antibody to human Nuclear Membrane. Validated for ICC and IF.
Target
Human Nuclear Membrane
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
AE-5
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free.
Applications
  • ICC (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Applications tested for the base form of this product only
Immunogen
Nuclei of myeloid leukemia biopsy cells were used as the immunogen for the Nuclear Membrane antibody.
Specificity
Human Nuclear Membrane
Usage
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the nuclear membrane expressed in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.,The nuclear envelope (also known as the perinuclear envelope, nuclear membrane, nucleolemma or karyotheca) is the double membrane of the nucleus that encloses genetic material in eukaryotic cells. It separates the contents of the nucleus (DNA in particular) from the cytosol (cytoplasm). Numerous nuclear pores are present on the nuclear envelope to facilitate and regulate the exchange of materials (for example, proteins and RNA) between the nucleus and the cytoplasm. The space between the two membranes that make up the nuclear envelope is called the perinuclear space (also called the perinuclear cisterna), and is usually about 20 - 40 nm wide. Each of the two membranes is composed of a lipid bilayer. The outer membrane is continuous with the rough endoplasmic reticulum. The inner membrane is erected upon the nuclear lamina, a network of intermediate filaments made of lamin, that plays a role in mitosis and meiosis. The type of lamins present are A, B1, B2, and C. The nuclear envelope may also play a role in the disposition of chromatin inside the nucleus. The lamina acts as a site of attachment for chromosomes. It also acts like a shield for the nucleus. During prophase in mitosis, the chromatids begin condensing to form chromosomes, and the nuclear envelope begins to disintegrate. During metaphase, the nuclear envelope is completely disintegrated, and the chromosomes can be pulled apart as chromatids by the spindle fibers. Optimal dilution of the Nuclear Membrane antibody should be determined by the researcher.
Presentation
0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/20/2019