NSF antibody LS-C17877 is an unconjugated mouse monoclonal antibody to NSF from human, mouse, rat and other species. Validated for IP and WB.
Bovine, Human, Monkey, Mouse, Rat, Dog, Hamster, Pig, Rabbit, Sheep, Chicken
(tested or 100% immunogen sequence identity)
NSF antibody was raised against recombinant bovine alpha SNAP proteins expressed in E. coli.
Detects a doublet of 35 and 36kD proteins, corresponding to the apparent molecular mass of alpha/beta SNAP on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, hamster, insect (tick), monkey, porcine, rabbit and sheep. beta SNAP is expressed in brain, while alpha SNAP has a more ubiquitous tissue distribution. Species Reactivity: human, mouse, rat, bovine, canine, chicken, hamster, insect (tick), monkey, porcine, rabbit, sheep.
Western Blot (Colorimetric): 1.5 ug/ml. Western Blot (ECL). Immunoprecipitation. Positive control: Rat Brain Tissue Extract, Mouse Brain Tissue Extract.
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.