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Catalog Number Size Price
LS-C662162-10 10 µg $318 
LS-C662162-100 100 µg $470 
NPAS2 Antibody - Western blot analysis of NPAS2 using anti-NPAS2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate,Lane 3: human COLO-320 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPAS2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NPAS2 at approximately 92KD. The expected band size for NPAS2 is at 92KD.

Polyclonal Rabbit anti‑Human NPAS2 Antibody (WB) LS‑C662162

Polyclonal Rabbit anti‑Human NPAS2 Antibody (WB) LS‑C662162

Antibody:
NPAS2 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662162-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NPAS2 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NPAS2 antibody LS-C662162 is an unconjugated rabbit polyclonal antibody to human NPAS2. Validated for WB.
Target
Human NPAS2
Synonyms
NPAS2 | BHLHe9 | Member of PAS protein 4 | Member of PAS superfamily 4 | Neuronal PAS2 | PASD4 | MOP4 | Neuronal PAS domain protein 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human NPAS2 recombinant protein (Position: D474-Q578). Human NPAS2 shares 88.1% amino acid (aa) sequence identity with mouse NPAS2.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NPAS2
Q99743 NM_002518 NP_002509.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024