Human NGFR / CD271 / TNR16

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC271/3D7

Unconjugated. Also available conjugated with FITC, Biotin.

Protein A purified

Unmodified

Recombinant soluble human CD271.

Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.

• Flow Cytometry
• ELISA

Five x 105 cultured human Reh cells per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD271 antibody(ANC271/3D7) at 5 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.76 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08138 NCBI: NM_002507 NP_002498.1