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Catalog Number Size Price
LS-C746963-20 20 µl $277 
LS-C746963-50 50 µl $319 
LS-C746963-100 100 µl $397 
LS-C746963-200 200 µl $499 
NFKBIA / IKB Alpha / IKBA Antibody - Immunofluorescence analysis of HeLa cells using NFKBIA antibody. Blue: DAPI for nuclear staining.
NFKBIA / IKB Alpha / IKBA Antibody - Western blot analysis of extracts from normal (control) and NFKBIA knockout (KO) 293T cells, using NFKBIA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.
NFKBIA / IKB Alpha / IKBA Antibody - Western blot analysis of extracts of various cell lines, using NFKBIA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
NFKBIA / IKB Alpha / IKBA Antibody - Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug NFKBIA antibody. Western blot was performed from the immunoprecipitate using NFKBIA antibody at a dilition of 1:500.
NFKBIA / IKB Alpha / IKBA Antibody - Immunofluorescence analysis of HeLa cells using NFKBIA antibody. Blue: DAPI for nuclear staining.
NFKBIA / IKB Alpha / IKBA Antibody - Western blot analysis of extracts from normal (control) and NFKBIA knockout (KO) 293T cells, using NFKBIA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.
NFKBIA / IKB Alpha / IKBA Antibody - Western blot analysis of extracts of various cell lines, using NFKBIA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
NFKBIA / IKB Alpha / IKBA Antibody - Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug NFKBIA antibody. Western blot was performed from the immunoprecipitate using NFKBIA antibody at a dilition of 1:500.
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Polyclonal Rabbit anti‑Human NFKBIA / IKB Alpha / IKBA Antibody (IF, WB) LS‑C746963

Polyclonal Rabbit anti‑Human NFKBIA / IKB Alpha / IKBA Antibody (IF, WB) LS‑C746963

Antibody:
NFKBIA / IKB Alpha / IKBA Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$277
LS-C746963-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NFKBIA / IKB Alpha / IKBA Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IKBA antibody LS-C746963 is an unconjugated rabbit polyclonal antibody to IKBA (NFKBIA / IKB Alpha) from human. It is reactive with human and mouse. Validated for IF, IP and WB.
Target
Human NFKBIA / IKB Alpha / IKBA
Synonyms
NFKBIA | IkB-alpha | IKBA | IkappaBalpha | MAD3 | NF-kappa-B inhibitor alpha | NFKBI | I-kappa-B-alpha | IKB Alpha | MAD-3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human NFKBIA (NP_065390.1). MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Applications
  • Immunofluorescence (1:20 - 1:50)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:20 - 1:50)
Usage
The predicted MW is 35kDa, while the observed MW by Western blot was 36kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKBIA / IKB Alpha / IKBA
P25963 NM_020529 NP_065390.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/18/2022