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Antibodies > NFAT4 / NFATC3 > LS-C801168
 
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Catalog Number Size Price
LS-C801168-100 100 µl (1 mg/ml) $379 
LS-C801168-200 200 µl (1 mg/ml) $421 
NFAT4 / NFATC3 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFAT4 / NFATC3 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NFAT4 / NFATC3 Antibody - Western blot analysis of NFAT4 phosphorylation expression in HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NFAT4 / NFATC3 Antibody - Western blot analysis of Phospho-NFAT4 (Ser165) expression in various lysates
NFAT4 / NFATC3 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFAT4 / NFATC3 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NFAT4 / NFATC3 Antibody - Western blot analysis of NFAT4 phosphorylation expression in HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NFAT4 / NFATC3 Antibody - Western blot analysis of Phospho-NFAT4 (Ser165) expression in various lysates
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Polyclonal Rabbit anti‑Human NFAT4 / NFATC3 Antibody (phospho‑Ser165, IF, WB) LS‑C801168

Polyclonal Rabbit anti‑Human NFAT4 / NFATC3 Antibody (phospho‑Ser165, IF, WB) LS‑C801168

Submit Review LSBio Guarantee
Antibody:
NFAT4 / NFATC3 Rabbit anti-Human Polyclonal (pSer165) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801168-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NFAT4 / NFATC3 Rabbit anti-Human Polyclonal (pSer165) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NFATC3 antibody LS-C801168 is an unconjugated rabbit polyclonal antibody to NFATC3 (NFAT4) (pSer165) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human NFAT4 / NFATC3
Synonyms
NFATC3 | NF-AT4 | NFATX | NF-AT4c | NFAT4 | NF-ATc3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human NFAT4 around the phosphorylation site of Serine 165.
Epitope
pSer165
Specificity
Phospho-NFAT4 (Ser165) Antibody detects endogenous levels of NFAT4 only when phosphorylated at Serine 165.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFAT4 / NFATC3
Q12968 NM_004555 NP_004546.1

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NFAT4 / NFATC3 Antibody - Western blot of NFAT4 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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