Target
Human NF-L / NEFL
Synonyms
NEFL | 68 kDa neurofilament protein | CMT1F | CMT2E | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-EKQLQELEDKQNAD, from the internal region of the protein sequence according to NP_006149.2.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 68-70kDa band observed in lysates of Neuroblastoma cell line KELLY (calculated MW of 61.5kDa according to NP_006149.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee