Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C2722-250 250 µl $335 
NEFM / NF-M Antibody - View of mixed neuron/glial cultures stained with NEFM / NF-M Antibody Neurofilament - Medium (NF-M) Antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
NEFM / NF-M Antibody - 160kDa Neurofilament Medium Antibody - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M Antibody Neurofilament - Medium (NF-M) Antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
NEFM / NF-M Antibody - View of mixed neuron/glial cultures stained with NEFM / NF-M Antibody Neurofilament - Medium (NF-M) Antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
NEFM / NF-M Antibody - 160kDa Neurofilament Medium Antibody - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M Antibody Neurofilament - Medium (NF-M) Antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
1 of 2
2 of 2

Polyclonal NEFM / NF‑M Antibody (C‑Terminus, IF, WB) LS‑C2722

Polyclonal NEFM / NF‑M Antibody (C‑Terminus, IF, WB) LS‑C2722

Antibody:
Chicken Polyclonal to Rat NEFM / NF-M
Application:
ICC, IF, WB
Reactivity:
Rat, Human, Mouse, Cat, Chicken, Fish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C2722-250
250 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Chicken Polyclonal to Rat NEFM / NF-M
Application:
ICC, IF, WB
Reactivity:
Rat, Human, Mouse, Cat, Chicken, Fish
Format:
Unconjugated, Unmodified

Specifications

Description
NF-M antibody LS-C2722 is an unconjugated chicken polyclonal antibody to NF-M (NEFM ) from human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Rat NEFM / NF-M
Synonyms
NEFM | 160 kDa neurofilament protein | NEF3 | NF-M | NFM | Neurofilament 3
Host
Chicken
Reactivity
Rat, Human, Mouse, Cat, Chicken, Fish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence (1:2000)
  • Western blot (1:10000)
Immunogen
The immunogen used to raise this particular antibody was to the C-Terminus extension of rat NF-M, the so-called KE segment, was expressed in bacteria and purified from inclusion bodies by dissolving in 6 M urea followed by preparative gel electrophoresis
Epitope
C-Terminus
Specificity
Neurofilament - Medium.
Presentation
Affinity purified antiserum.
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEFM / NF-M
P07197 NM_005382 NP_005373.2

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 2/16/2020