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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C164777-400 400 µl $325 
NCL / Nucleolin Antibody - Fluorescent confocal image of U251 cell stained with NCL Antibody (Center P291). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.
NCL / Nucleolin Antibody - NCL Antibody (Center P291) western blot of human placenta tissue lysates (35 ug/lane). The NCL antibody detected the NCL protein (arrow).
NCL / Nucleolin Antibody - Fluorescent confocal image of U251 cell stained with NCL Antibody (Center P291). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.
NCL / Nucleolin Antibody - NCL Antibody (Center P291) western blot of human placenta tissue lysates (35 ug/lane). The NCL antibody detected the NCL protein (arrow).
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NCL / Nucleolin Antibody (aa276‑304) LS‑C164777

NCL / Nucleolin Antibody (aa276‑304) LS‑C164777

Antibody:
Rabbit Polyclonal to Human NCL / Nucleolin
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C164777-400
400 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NCL / Nucleolin
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nucleolin antibody LS-C164777 is an unconjugated rabbit polyclonal antibody to human Nucleolin (NCL ). Validated for IF and WB.
Target
Human NCL / Nucleolin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:1000)
Epitope
aa276-304
Specificity
This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human NCL.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCL / Nucleolin
P19338 NM_005381 NP_005372.2

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Requested From: United States
Date Requested: 11/12/2019