NANOG | Nanog homeobox | NANOGP8 | HNanog | Homeobox protein NANOG
(tested or 100% immunogen sequence identity)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Peptide with sequence C-DLEVNLEATRESHAH, from the internal region of the protein sequence according to NP_082292.1 .
- Western blot (0.5 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 34.6kDa according to NP_082292.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zbinden et al, EMBO J. 2010 A ug 4;29(15):2659-74. PMID: 20581802). Recommended concentration: 0.5-2 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.