Dog Na+/K+ ATPase

Chicken

Dog, Canine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

ATPase, Sodium, Potassium [Canine Kidney]

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney]. Cross reactivity against ATPase, Sodium, Potassium from other tissues and species may occur but have not been specifically determined.

• Western blot (1:500 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:20000)

Suitable for immunoblotting (western or dot blot), ELISA, conjugation and most immunological methods requiring high titer and specificity. This product has been assayed by western blot and was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kD and beta at 50 kD) as well as glycosylated and non-glycosylated enzyme forms. A dilution of 1:100 to 1:500 is suggested for immunoblotting followed by reaction with Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) catalog no. LS-C60281 and ABTS as a substrate for 30 minutes at room temperature. This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) catalog no. LS-C60281 and ABTS as a substrate for 30 minutes at room temperature.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 1 mL of deionized water.

Store vial at 4°C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.

For research use only. Intended for use by laboratory professionals.

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