Bovine Myosin, Smooth Muscle
Smooth muscle myosin
(tested or 100% immunogen sequence identity)
Bovine tracheal smooth muscle myosin.
Recognizes the heavy chain of myosin II found in vertebrate smooth muscle, a non-striated muscle present in the walls of hollow organs, the vasculature, the respiratory and gastrointestinal tracts, uterus, bladder and eye. The structure of myosin II is fundamentally the same in all muscle types and consists of two heavy chains, each bound by 2 distinct, essential and regulatory light chains. The globular motor domain located at the N-terminal domain of the heavy chains, catalyzes ATP hydrolysis and interacts with actin, whilst the heptad repeat sequences of the heavy chain tail domains promote dimerization, forming an alpha-helical coiled coil at the C-terminal domain. Does not cross-react with myosin of skeletal or non-muscle origin and can be used to specifically stain cultured smooth muscle cells and identify vascular and other smooth muscle types in tissue sections.