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Antibodies > MYO18B / Myosin 18B > LS-C757778
 
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Catalog Number Size Price
LS-C757778-200 200 µl $393 
MYO18B / Myosin 18B Antibody - MYO18B Antibody staining MYO18B in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
MYO18B / Myosin 18B Antibody - MYO18B Antibody staining MYO18B in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
MYO18B / Myosin 18B Antibody - Anti-MYO18B Antibody at 1:16000 dilution + Recombinant protein Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 285 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
MYO18B / Myosin 18B Antibody - Overlay histogram showing A431 cells stained with MYO18B Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MYO18B Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OJ192088) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2b (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
MYO18B / Myosin 18B Antibody - MYO18B Antibody staining MYO18B in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
MYO18B / Myosin 18B Antibody - MYO18B Antibody staining MYO18B in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
MYO18B / Myosin 18B Antibody - Anti-MYO18B Antibody at 1:16000 dilution + Recombinant protein Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 285 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
MYO18B / Myosin 18B Antibody - Overlay histogram showing A431 cells stained with MYO18B Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MYO18B Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OJ192088) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2b (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Monoclonal Mouse anti‑Human MYO18B / Myosin 18B Antibody (clone 1683CT270.39.50, IHC, WB) LS‑C757778

Monoclonal Mouse anti‑Human MYO18B / Myosin 18B Antibody (clone 1683CT270.39.50, IHC, WB) LS‑C757778

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Antibody:
MYO18B / Myosin 18B Mouse anti-Human Monoclonal (1683CT270.39.50) Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757778-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MYO18B / Myosin 18B Mouse anti-Human Monoclonal (1683CT270.39.50) Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Myosin 18B antibody LS-C757778 is an unconjugated mouse monoclonal antibody to human Myosin 18B (MYO18B). Validated for ELISA, Flow, IHC and WB.
Target
Human MYO18B / Myosin 18B
Synonyms
MYO18B | BK125H2.1 | Myosin 18B | Myosin XVIIIB | Myosin-XVIIIb | Unconventional myosin-XVIIIb
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
1683CT270.39.50
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
This MYO18B antibody is generated from a mouse immunized with recombinant protein from human MYO18B.
Applications
  • IHC - Paraffin (1:25)
  • Western blot (1:16000)
  • Flow Cytometry (1:25)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 285.2 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYO18B / Myosin 18B
Q8IUG5 NM_032608 NP_115997.5

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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