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Catalog Number Size Price
LS-C154222-100 100 µg (1 mg/ml) $335 
Myc Tag Antibody - Anti-Myc epitope tag Antibody - Western Blot. Anti-Myc epitope tag polyclonal antibody detects ~100 kD CARBOXY terminal linked Myc-tagged recombinant protein present in ~35 ug of lysate by western blot. Carboxy terminal linked Myc recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.
Myc Tag Antibody - Anti-Myc epitope Tag Antibody - Western Blot. Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by western blot. Polyclonal rabbit host anti-Myc epitope tag antibody was diluted to 1.0 ug/ml to detect either recombinant protein. 4-20% gradient gels were used to resolve the proteins by SDS-PAGE. The proteins were transferred to nitrocellulose using standard methods. After blocking, the membranes were probed with the primary antibody overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 45 min at room temperature (Green, 800 nm channel). Pre-stained molecular weight markers are also shown (lane M, Red, 700 nm channel). LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Myc Tag Antibody - Anti-Myc epitope tag Antibody - Western Blot. Anti-Myc epitope tag polyclonal antibody detects ~100 kD CARBOXY terminal linked Myc-tagged recombinant protein present in ~35 ug of lysate by western blot. Carboxy terminal linked Myc recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.
Myc Tag Antibody - Anti-Myc epitope Tag Antibody - Western Blot. Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by western blot. Polyclonal rabbit host anti-Myc epitope tag antibody was diluted to 1.0 ug/ml to detect either recombinant protein. 4-20% gradient gels were used to resolve the proteins by SDS-PAGE. The proteins were transferred to nitrocellulose using standard methods. After blocking, the membranes were probed with the primary antibody overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 45 min at room temperature (Green, 800 nm channel). Pre-stained molecular weight markers are also shown (lane M, Red, 700 nm channel). LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Myc Tag Antibody (aa410‑419) LS‑C154222

Myc Tag Antibody (aa410‑419) LS‑C154222

Antibody:
Rabbit Polyclonal to Myc Tag
Application:
IHC, WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C154222-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Myc Tag
Application:
IHC, WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
Myc Tag antibody LS-C154222 is an unconjugated rabbit polyclonal antibody to Myc Tag. Validated for ELISA, IHC and WB.
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:500 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.
Epitope
aa410-419
Specificity
This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Usage
Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/11/2019