ELISA: In a simple ELISA with 1 ug of antigen coated per well, the 50% OD was observed at 1/20000. Under the same conditions, the end point was observed at >1/400000. Alkaline phosphatase conjugated anti-sheep was used for detection. All incubations at 18°C using pNPP substrate, with OD measurements at 405 nm.
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
For research use only. Intended for use by laboratory professionals.
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