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Antibodies > MtTFA / TFAM > LS-C407664
 
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Catalog Number Size Price
LS-C407664-10 10 µg $318 
LS-C407664-100 100 µg $470 
MtTFA / TFAM Antibody - mtTFA antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
MtTFA / TFAM Antibody - IF analysis of mtTFA using anti-mtTFA antibody mtTFA was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-mtTFA Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MtTFA / TFAM Antibody - Western blot analysis of mtTFA using anti-mtTFA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24KD. The expected band size for mtTFA is at 29KD.
MtTFA / TFAM Antibody - Western blot analysis of mtTFA using anti-mtTFA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24KD. The expected band size for mtTFA is at 29KD.
MtTFA / TFAM Antibody - mtTFA antibody Western blot. All lanes: Anti mtTFA at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: HEPA Whole Cell Lysate at 40 ug. Lane 3: Human Placenta Tissue Lysate at 50 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Lane 6: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 29 kD. Observed band size: 29 kD.
MtTFA / TFAM Antibody - Flow Cytometry analysis of SiHa cells using anti-mtTFA antibody. Overlay histogram showing SiHa cells stained with anti-mtTFA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-mtTFA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MtTFA / TFAM Antibody - mtTFA antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
MtTFA / TFAM Antibody - IF analysis of mtTFA using anti-mtTFA antibody mtTFA was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-mtTFA Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MtTFA / TFAM Antibody - Western blot analysis of mtTFA using anti-mtTFA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24KD. The expected band size for mtTFA is at 29KD.
MtTFA / TFAM Antibody - Western blot analysis of mtTFA using anti-mtTFA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24KD. The expected band size for mtTFA is at 29KD.
MtTFA / TFAM Antibody - mtTFA antibody Western blot. All lanes: Anti mtTFA at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: HEPA Whole Cell Lysate at 40 ug. Lane 3: Human Placenta Tissue Lysate at 50 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Lane 6: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 29 kD. Observed band size: 29 kD.
MtTFA / TFAM Antibody - Flow Cytometry analysis of SiHa cells using anti-mtTFA antibody. Overlay histogram showing SiHa cells stained with anti-mtTFA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-mtTFA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human MtTFA / TFAM Antibody (aa214‑241, IHC, WB) LS‑C407664

Polyclonal Rabbit anti‑Human MtTFA / TFAM Antibody (aa214‑241, IHC, WB) LS‑C407664

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Antibody:
MtTFA / TFAM Rabbit anti-Human Polyclonal (aa214-241) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407664-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MtTFA / TFAM Rabbit anti-Human Polyclonal (aa214-241) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TFAM antibody LS-C407664 is an unconjugated rabbit polyclonal antibody to TFAM (MtTFA) (aa214-241) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human MtTFA / TFAM
Synonyms
TFAM | MTTF1 | TCF-6 | TCF6 | TCF6L1 | Transcription factor 6-like 2 | Transcription factor 6-like 3 | TCF6L2 | TCF6L3 | Transcription factor 6 | Transcription factor 6-like 1 | MTTFA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human mtTFA (214-241 aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids.
Epitope
aa214-241
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
WB: The detection limit for mtTFA is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MtTFA / TFAM
Q00059 NM_003201 NP_003192.1

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Featured Products

MtTFA / TFAM Antibody - Mouse Testis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
MtTFA / TFAM Antibody (aa169‑218) LS‑C30495
Species: Mouse
Applications: IHC, IHC - Paraffin, Western blot
MtTFA / TFAM Antibody - Anti-TFAM antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
MtTFA / TFAM Antibody (aa75‑124) LS‑B1571
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
MtTFA / TFAM Antibody - Anti-TFAM antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
MtTFA / TFAM Antibody (aa151‑200) LS‑B6950
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot
MtTFA / TFAM Antibody - Anti-MtTFA / TFAM antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ MtTFA / TFAM Antibody (clone 18G102B2E11) LS‑B9989
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
MtTFA / TFAM Antibody - Anti-TFAM antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ MtTFA / TFAM Antibody (aa131‑180) LS‑B5669
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
MtTFA / TFAM Antibody - Western blot of recombinant MtTFA / TFAM. This image was taken for the unconjugated form of this product. Other forms have not been tested.
MtTFA / TFAM Antibody (aa43‑246) LS‑C296734
Species: Human
Applications: Western blot
See all MtTFA / TFAM Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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