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Catalog Number Size Price
LS-C744825-25 25 µl (1 mg/ml) $275 
MSLN / Mesothelin Antibody - Immunohistochemistry using the anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN.
MSLN / Mesothelin Antibody - Immunohistochemistry of Mouse anti-Mesothelin antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Mesothelin antibody at 15 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Mesothelin as precipitated red signal with hematoxylin purple nuclear counterstain.
MSLN / Mesothelin Antibody - Western blotting using the anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1µg/ml followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate.
MSLN / Mesothelin Antibody - Immunohistochemistry using the anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN.
MSLN / Mesothelin Antibody - Immunohistochemistry of Mouse anti-Mesothelin antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Mesothelin antibody at 15 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Mesothelin as precipitated red signal with hematoxylin purple nuclear counterstain.
MSLN / Mesothelin Antibody - Western blotting using the anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1µg/ml followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate.
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Monoclonal Mouse anti‑Human MSLN / Mesothelin Antibody (clone MN‑1, IHC, WB) LS‑C744825

Monoclonal Mouse anti‑Human MSLN / Mesothelin Antibody (clone MN‑1, IHC, WB) LS‑C744825

Antibody:
MSLN / Mesothelin Mouse anti-Human Monoclonal (MN-1) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C744825-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
MSLN / Mesothelin Mouse anti-Human Monoclonal (MN-1) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Mesothelin antibody LS-C744825 is an unconjugated mouse monoclonal antibody to human Mesothelin (MSLN). Validated for ELISA, IHC and WB.
Target
Human MSLN / Mesothelin
Synonyms
MSLN | CAK1 antigen | MPF | SMRP | Mesothelin | CAK1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
MN-1
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Applications
  • IHC (1:100)
  • Western blot (1:1000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MSLN / Mesothelin
Q13421 NM_013404 NP_037536.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021