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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MRGPRX2 / MRGX2 Antibody (C‑Terminus) IHC‑plus™ LS‑A6647 Best Seller
MRGX2 antibody LS-A6647 is an unconjugated rabbit polyclonal antibody to human MRGX2 (MRGPRX2). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A6647-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment
Description
MRGX2 antibody LS-A6647 is an unconjugated rabbit polyclonal antibody to human MRGX2 (MRGPRX2). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human MRGPRX2 / MRGX2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 - 20 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MRGPRX2 / MRGX2 antibody was raised against synthetic 16 amino acid peptide from C-terminal cytoplasmic domain of human MRGPRX2 / MRGX2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan (94%); Gibbon, Monkey (88%).
Blocking Peptide
LS-E29162 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A6647. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human MRGPRX2 / MRGX2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A6647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A6647 was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MRGPRX2 / MRGX2
Q96LB1 NM_054030 NP_473371.1

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Western blot. All lanes: MRGPRX2 antibody at 5 ug/ml. Lane 1: 293T whole cell lysate. Lane 2: Mouse liver tissue. Lane 3: MCF7 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa.
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Images

Immunohistochemistry

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Requested From: United States
Date Requested: 12/13/2018
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