Human Kappa Light Chain

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

HP6053 + L1C1

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Purified human Ig kappa chain (HP6053) and human B-lymphoma cells (L1C1) were used as the immunogens for this Kappa Light Chain antibody cocktail.

Human Kappa Light Chain

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)

This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. The optimal dilution of the Kappa Light Chain antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.