Human IgG1 Fc

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NI 132 (HP 6186)

Biotin. Also available Unconjugated or conjugated with FITC, HRP, TRITC.

Purified IgG

Unmodified

Highly purified monoclonal IgG1 isolated from pooled human serum.

Human IgG1, subclass specific.

• IHC
• IHC - Paraffin
• ICC
• ELISA
• Dot Blot
• Applications tested for the base form of this product only

The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40. in ELISA from 1:100 to 1:2000. in Western blotting from 1:200 to 1:4000. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.