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Antibodies > MMP10 > LS-C662203
 
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Catalog Number Size Price
LS-C662203-10 10 µg $318 
LS-C662203-100 100 µg $470 
MMP10 Antibody - Immunohistochemistry - Anti-MMP10 Picoband Antibody
MMP10 Antibody - Immunohistochemistry - Anti-MMP10 Picoband Antibody
MMP10 Antibody - IF analysis of MMP10 using anti-MMP10 antibody. MMP10 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MMP10 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. isualize using a fluorescence microscope and filter sets appropriate for the label used.
MMP10 Antibody - Western blot analysis of MMP10 using anti-MMP10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP10 at approximately 54KD. The expected band size for MMP10 is at 54KD.
MMP10 Antibody - Western blot analysis of MMP10 using anti-MMP10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: mouse cardiac muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP10 at approximately 54KD. The expected band size for MMP10 is at 54KD.
MMP10 Antibody - Western blot - Anti-MMP10 Picoband Antibody
MMP10 Antibody - Immunohistochemistry - Anti-MMP10 Picoband Antibody
MMP10 Antibody - Immunohistochemistry - Anti-MMP10 Picoband Antibody
MMP10 Antibody - IF analysis of MMP10 using anti-MMP10 antibody. MMP10 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MMP10 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. isualize using a fluorescence microscope and filter sets appropriate for the label used.
MMP10 Antibody - Western blot analysis of MMP10 using anti-MMP10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP10 at approximately 54KD. The expected band size for MMP10 is at 54KD.
MMP10 Antibody - Western blot analysis of MMP10 using anti-MMP10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: mouse cardiac muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP10 at approximately 54KD. The expected band size for MMP10 is at 54KD.
MMP10 Antibody - Western blot - Anti-MMP10 Picoband Antibody
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Polyclonal Rabbit anti‑Human MMP10 Antibody (IHC, WB) LS‑C662203

Polyclonal Rabbit anti‑Human MMP10 Antibody (IHC, WB) LS‑C662203

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Antibody:
MMP10 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662203-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MMP10 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MMP10 antibody LS-C662203 is an unconjugated rabbit polyclonal antibody to human MMP10. Validated for ELISA, IHC and WB.
Target
Human MMP10
Synonyms
MMP10 | Matrix metalloproteinase-10 | SL-2 | STMY2 | Stromelysin 2 | Stromelysin-2 | MMP-10 | Transin 2 | Transin-2 | Stromelysin2 | Matrix metalloprotease 10
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human MMP10 recombinant protein (Position: Y239-C476).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MMP10
P09238 NM_002425 NP_002416.1

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Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
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Species: Human
Applications: Western blot, ELISA
MMP10 Antibody - Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MMP10. The membrane blot was probed with antiMMP10 primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein MMP10 from E.coli cell (55kDa).
MMP10 Antibody (Preservative Free) LS‑C343495
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Applications: Western blot, ELISA
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MMP10 Antibody (aa394‑423) LS‑C327846
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
MMP10 Antibody - Immunohistochemical analysis of MMP10 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MMP10 Antibody (C‑Terminus) LS‑C356187
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry
MMP10 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
MMP10 Antibody (aa394‑423) LS‑C100440
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
See all MMP10 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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