Human MLANA / Melan-A

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

A103

Unconjugated

Protein G affinity chromatography

Unmodified

A recombinant protein was used as immunogen.

• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Due to differences in protocols and secondary antibody used, the Melan-A antibody may require titration for optimal performance. FFPE staining is enhanced by boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Prediluted IHC only format: incubate for 30 min at RT

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16655 NCBI: NM_005511 NP_005502.1