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Catalog Number	Size	Price
LS-C357429-10	10 µg	$318
LS-C357429-100	100 µg (0.5 mg/ml)	$470

Mitofusin 2 / MFN2 Antibody - Western blot analysis of Mitofusin 2 using anti-Mitofusin 2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mitofusin 2 at approximately 86KD. The expected band size for Mitofusin 2 is at 86KD.

Mitofusin 2 / MFN2 Antibody - Mitofusin-2 antibody Western blot. All lanes: Anti Mitofusin-2 at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: A549 Whole Cell Lysate at 40 ug. Predicted band size: 86 kD. Observed band size: 86 kD.

Mitofusin 2 / MFN2 Antibody - Mitofusin-2 antibody Western blot. All lanes: Anti Mitofusin-2 at 0.5 ug/ml. WB: Recombinant Human Mitofusin-2 Protein 0.5ng. Predicted band size: 45 kD. Observed band size: 45 kD.

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Polyclonal Rabbit anti‑Human Mitofusin 2 / MFN2 Antibody (aa601‑757, WB) LS‑C357429

Name: Anti-Mitofusin 2 / MFN2 Antibody | Rabbit anti-Human Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C357429-10
Price: 318.0000 USD
Availability: InStock

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Note: This antibody replaces LS-C388861

Antibody:

Mitofusin 2 / MFN2 Rabbit anti-Human Polyclonal (aa601-757) Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C357429-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

Mitofusin 2 / MFN2 Rabbit anti-Human Polyclonal (aa601-757) Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

MFN2 antibody LS-C357429 is an unconjugated rabbit polyclonal antibody to human MFN2 (Mitofusin 2) (aa601-757). Validated for WB.

Target

Human Mitofusin 2 / MFN2

Synonyms

See All Mitofusin 2 / MFN2 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human Mitofusin 2 recombinant protein (Position: V601-R757). Human Mitofusin 2 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat Mitofusin 2, respectively.

Epitope

aa601-757

Specificity

Ubiquitous; expressed at low level. Highly expressed in heart and kidney. .

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About Mitofusin 2 / MFN2

Uniprot: O95140 NCBI: NM_014874 NP_055689.1