Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-2 (15.0 ng/ml), a concentration of 0.52-0.9 ug/ml of this antibody is required. Sandwich ELISA: To detect Murine MIP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with LifeSpan's Biotinylated Anti-Murine MIP-2 (LS-C104503
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-2. Western Blot: To detect Murine MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine MIP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.