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MHC Class II IA/IE Antibody (clone M5/114.15.2, Biotin) LS‑C106140
Note: This antibody replaces LS-C47990
IE antibody LS-C106140 is a biotin-conjugated rat monoclonal antibody to mouse IE (MHC Class II IA). Validated for Flow.
Catalog
Size
Price
LS-C106140-50
50 µg
$215
LS-C106140-100
100 µg
$275
LS-C106140-500
500 µg
$355

Popular MHC Class II IA/IE Products

Staining of C57Bl/6 splenocytes with 0.06 ug of Purified Rat IgG2b isotype control (open histogram) or 0.06 ug of Purified M5/114.15.2 (colored histogram) followed by FITC Anti-Rat IgG. Total viable cells were used for analysis.
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Staining of C57Bl/6 splenocytes with FITC anti-CD45R/B220 (LS-C105879) and 0.0075 ug of PE M5/114.15.2. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with FITC anti-mouse CD3e (145-2C11) (LS-C105774) and 0.015 ug of APC Rat IgG2b Isotype Control (left) or 0.015 ug of APC anti-mouse Class II (M5/114.15.2) (right). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Antibody
Species: Mouse
Applications: Immunofluorescence, Flow Cytometry
Antibody
Species: Mouse
Applications: Flow Cytometry

Product Description

IE antibody LS-C106140 is a biotin-conjugated rat monoclonal antibody to mouse IE (MHC Class II IA). Validated for Flow.

Specifications

Target
Mouse MHC Class II IA/IE
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [M5/114.15.2]
Conjugations
Biotin
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
Mouse MHC Class II IA/IE
Usage
The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspension and can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Flow Cytometry

Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of C57Bl/6 splenocytes with 0.015 ug of biotin rat IgG2b isotype control (open histogram), or 0.015 ug of biotin M5/114.15.2 followed by SAv-PE (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 8/20/2018

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