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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MHC Class II IA/IE Antibody (clone M5/114.15.2, APC) LS‑C407523
IE antibody LS-C407523 is an APC-conjugated rat monoclonal antibody to mouse IE (MHC Class II IA). Validated for Flow.
Catalog
Size
Price
LS-C407523-25
25 µg
$255
LS-C407523-100
100 µg (0.2 mg/ml)
$465

Popular MHC Class II IA/IE Products

Staining of C57Bl/6 splenocytes with 0.06 ug of Purified Rat IgG2b isotype control (open histogram) or 0.06 ug of Purified M5/114.15.2 (colored histogram) followed by FITC Anti-Rat IgG. Total viable cells were used for analysis.
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Staining of C57Bl/6 splenocytes with FITC anti-CD45R/B220 (LS-C105879) and 0.0075 ug of PE M5/114.15.2. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with FITC anti-mouse CD3e (145-2C11) (LS-C105774) and 0.015 ug of APC Rat IgG2b Isotype Control (left) or 0.015 ug of APC anti-mouse Class II (M5/114.15.2) (right). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Antibody
Species: Mouse
Applications: Immunofluorescence, Flow Cytometry
Antibody
Species: Mouse
Applications: Flow Cytometry

Product Description

MHC Class II IA/IE Antibody (clone M5/114.15.2, APC) for Flow LS-C407523

Specifications

Target
Mouse MHC Class II IA/IE
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [M5/114.15.2]
Conjugations
Allophycocyanin
Purification
Purified
Modifications
Unmodified
Applications
  • Flow Cytometry (0.5 µg/10E6 cells)
Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used <= 0.5 ug /10e6 cells in 100 ul). Since applications vary, the appropriate dilutions must be determined for individual use. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light. Store undiluted.
Restrictions
For research use only.

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Images

Flow Cytometry

C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.
C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.
C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.
C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.
C57BL/6 mouse splenocytes stained with APC anti-mouse MHC class II. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/19/2018

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