Target
Rat MHC Class II I-Ab/s
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
R. Phycoerythrin
Purification
Ion exchange chromatography
Immunogen
Rat thymocyte membrane glycoproteins
Specificity
Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee