MGO / Methylglyoxal

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Unmodified

Methylglyoxal-KLH-adduct

• IHC - Paraffin (1 µg/ml)
• IHC - Frozen (1 µg/ml)
• Western blot (1 µg/ml)

The antibody 6B is suitable for the detection of MG-derived AGE especially of Arg-Pyrimidine in tissues and tissue extracts. Special focus will be put on the investigation of arterial sclerosis and protein deposits in the brain, renals, retina and lens. Methylglyoxal (MG) is a cytotoxic metabolite, which is synthesized as a side product of glycolysis. In diabetic hyperglycaemia accumulation of MG and irreversible binding to cellular proteins especially Arg- and Lys-residues may occur. This leads to the formation of "Advanced Glycation End Products" (AGE). The formation of MG-derived AGE contributes to several pathological conditions including artherosclerosis, Alzheimer's disease and complications in diabetes (blindness, neuropathy, nephropathy). The glyoxalase-1-system (GLO1) is stimulated in different tumours (Colon-, Mamma- and Prostate-Ca) and correlates with the very fast formation of MG-adducts in TNF-stimulated Apoptosis. This leads to the conclusion that in those cases AGE products are formed through enzymatic reactions. Methylglyoxal (MG)-Adducts, primarily Arg-Pyrimidine (N?-(4-Carboxy-4,6-Dimethyl-5,6-Dihydroxy-1,4,5,6-Tetrahydropyrimidine-2-yl)-L-Ornithine), cross-reactivity with Tetrahydropyrimidine and 5-Hydro-5-Methylimidazolone app. 0.1% in ELISA, Immidazolysine and (Carboxyethyl)lysine <0.1%.

10 mM PBS, 0.5% BSA, 0.1% sodium azide

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

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