Human MFGE8 /Lactadherin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

EDM45

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free, Ready-to-Use.

Delipidated human milk fat globule membrane preparation.

Human MFGE8 /Lactadherin

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-EA. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development. Optimal dilution of the Lactadherin antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q08431 NCBI: NM_005928 NP_005919.2