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Catalog Number Size Price
LS-C313081-10 10 µg $318 
LS-C313081-100 100 µg $470 
MFGE8 /Lactadherin Antibody - MFGE8 / MFG-E8 antibody. IHC(P): Human Breast Cancer Tissue.
MFGE8 /Lactadherin Antibody - Western blot analysis of MFGE8 using anti-MFGE8 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFGE8 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MFGE8 at approximately 66-75KD. The expected band size for MFGE8 is at 43KD.
MFGE8 /Lactadherin Antibody - WB of MFGE8 / MFG-E8 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant human MEGF8, 50.2KD. (162aa tag+ S28-A317). Lane 1: Recombinant Human MEGF8 Protein 10ng. Lane 2: Recombinant Human MEGF8 Protein 5ng. Lane 3: Recombinant Human MEGF8 Protein 2.5ng. Lane 4: Recombinant Human MEGF8 Protein 1.25ng.
MFGE8 /Lactadherin Antibody - MFGE8 / MFG-E8 antibody. IHC(P): Human Breast Cancer Tissue.
MFGE8 /Lactadherin Antibody - Western blot analysis of MFGE8 using anti-MFGE8 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFGE8 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MFGE8 at approximately 66-75KD. The expected band size for MFGE8 is at 43KD.
MFGE8 /Lactadherin Antibody - WB of MFGE8 / MFG-E8 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant human MEGF8, 50.2KD. (162aa tag+ S28-A317). Lane 1: Recombinant Human MEGF8 Protein 10ng. Lane 2: Recombinant Human MEGF8 Protein 5ng. Lane 3: Recombinant Human MEGF8 Protein 2.5ng. Lane 4: Recombinant Human MEGF8 Protein 1.25ng.
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Polyclonal Rabbit anti‑Human MFGE8 /Lactadherin Antibody (aa165‑180, IHC, WB) LS‑C313081

Polyclonal Rabbit anti‑Human MFGE8 /Lactadherin Antibody (aa165‑180, IHC, WB) LS‑C313081

Note: This antibody replaces LS-C388851
Antibody:
MFGE8 /Lactadherin Rabbit anti-Human Polyclonal (aa165-180) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313081-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MFGE8 /Lactadherin Rabbit anti-Human Polyclonal (aa165-180) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Lactadherin antibody LS-C313081 is an unconjugated rabbit polyclonal antibody to human Lactadherin (MFGE8) (aa165-180). Validated for IHC and WB.
Target
Human MFGE8 /Lactadherin
Synonyms
MFGE8 | Breast epithelial antigen BA46 | EDIL1 | HP47 | Lactadherin | Medin | MFGM | Milk fat globule-EGF factor 8 | HsT19888 | OAcGD3S | SPAG10 | Sperm associated antigen 10 | MFG-E8 | SED1 | Sperm surface protein hP47 | BA46 | HMFG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1(165-180 aa NGHEFDFIHDVNKKHK).
Epitope
aa165-180
Specificity
Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MFGE8 /Lactadherin
Q08431 NM_005928 NP_005919.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024