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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MFAP5 / MAGP2 Antibody (C-Terminus) LS-C18845

MAGP2 antibody LS-C18845 is an unconjugated rabbit polyclonal antibody to human MAGP2 (MFAP5). Validated for ELISA and WB.
Catalog
Size
Price
LS-C18845-100
100 µg (0.95 mg/ml)
$425
Description
MAGP2 antibody LS-C18845 is an unconjugated rabbit polyclonal antibody to human MAGP2 (MFAP5). Validated for ELISA and WB.
Target
Human MFAP5 / MAGP2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:250 - 1:1000)
  • ELISA (1:5000 - 1:20000)
Immunogen
MFAP5 / MAGP2 antibody was raised against synthetic peptide from human MFAP5.
Epitope
C-Terminus
Specificity
A region near the carboxy terminal end of human MAGP-2 protein.
Usage
This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20-30 kD in size corresponding to MAGP-2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MFAP5 / MAGP2
Q13361 NM_003480 NP_003471.1

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Images

Western blot

Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.
Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.

Western blot

Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.
Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.

Western blot

Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.
Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.

Western blot

Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.
Anti-MAGP-2 Antibody - Western Blot. Western blot of affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4C. Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 4/21/2019
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