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Catalog Number Size Price
LS-C662223-10 10 µg $318 
LS-C662223-100 100 µg $470 
MED14 Antibody - Western blot analysis of MED14 using anti-MED14 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED14 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED14 at approximately 160KD. The expected band size for MED14 is at 160KD.
MED14 Antibody - Western blot - Anti-MED14/Crsp2 Picoband Antibody
MED14 Antibody - Western blot analysis of MED14 using anti-MED14 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED14 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED14 at approximately 160KD. The expected band size for MED14 is at 160KD.
MED14 Antibody - Western blot - Anti-MED14/Crsp2 Picoband Antibody
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Polyclonal Rabbit anti‑Human MED14 Antibody (WB) LS‑C662223

Polyclonal Rabbit anti‑Human MED14 Antibody (WB) LS‑C662223

Antibody:
MED14 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662223-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MED14 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MED14 antibody LS-C662223 is an unconjugated rabbit polyclonal antibody to human MED14. Validated for ELISA and WB.
Target
Human MED14
Synonyms
MED14 | CSRP | CRSP2 | CXorf4 | CRSP150 | DRIP150 | EXLM1 | Human homolog of yeast RGR1 | HRGR1 | Mediator complex subunit 14 | TRAP170 | RGR1 homolog | ARC150 | CRSP complex subunit 2 | RGR1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human MED14 recombinant protein (Position: D181-D375).
Specificity
Ubiquitous.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MED14
O60244 NM_004229 NP_004220.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024