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Catalog Number Size Price
LS-C352495-100 100 µl (1 mg/ml) $299 
MCM5 Antibody - Immunohistochemical analysis of CDC46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MCM5 Antibody - Immunofluorescent analysis of CDC46 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MCM5 Antibody - Western blot analysis of CDC46 expression in HL60 (A); HeLa (B); HepG2 (C) whole cell lysates.
MCM5 Antibody - Immunohistochemical analysis of CDC46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MCM5 Antibody - Immunofluorescent analysis of CDC46 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MCM5 Antibody - Western blot analysis of CDC46 expression in HL60 (A); HeLa (B); HepG2 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human MCM5 Antibody (N‑Terminus, IHC, IF, WB) LS‑C352495

Polyclonal Rabbit anti‑Human MCM5 Antibody (N‑Terminus, IHC, IF, WB) LS‑C352495

Antibody:
MCM5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352495-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MCM5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
MCM5 antibody LS-C352495 is an unconjugated rabbit polyclonal antibody to MCM5 (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human MCM5
Synonyms
MCM5 | CDC46 | CDC46 homolog | p1-CDC46
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human CDC46.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of CDC46 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCM5
P33992 NM_006739 NP_006730.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024