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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MCHR1 Antibody (C‑Terminus) IHC‑plus™ LS‑A1567 Best Seller
MCHR1 antibody LS-A1567 is an unconjugated rabbit polyclonal antibody to MCHR1 from human, bovine, dog and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A1567-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular MCHR1 Products

Anti-SLC1 / MCHR1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1532 dilution 15-30 ug/ml.
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Anti-MCHR1 antibody LS-A1533 IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Antibody
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Applications: IHC, Western blot, Flow Cytometry, ELISA
MCHR1 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

MCHR1 antibody LS-A1567 is an unconjugated rabbit polyclonal antibody to MCHR1 from human, bovine, dog and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human MCHR1
Synonyms
MCHR1, G-protein coupled receptor 24, G protein-coupled receptor 24, GPR24, MCH-1R, MCH receptor 1, MCH-R1, MCH1R, Somatostatin-like receptor 1, MCH 1 receptor, MCHR, SLC-1, MCHR-1, SLC1
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MCHR1 antibody was raised against synthetic 18 amino acid peptide from C-terminus of human MCHR1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Bovine, Dog, Panda (100%); Mouse, Rat, Horse, Pig (94%); Hamster, Elephant (89%).
Blocking Peptide
LS-E28332 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1567. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human MCHR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A1567 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1567 was determined to be 10-18 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MCHR1
MCHR1, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin. Q99705 NM_005297 NP_005288.3


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Immunohistochemistry

Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-MCHR1 antibody LS-A1567 IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/17/2018

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