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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP2K5 / MEK5 Antibody IHC‑plus™ LS‑B15216
Note: This antibody replaces LS-C500775
MEK5 antibody LS-B15216 is an unconjugated rabbit polyclonal antibody to MEK5 (MAP2K5) from human, mouse and rat. Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B15216-50
50 µg
$425
Description
MEK5 antibody LS-B15216 is an unconjugated rabbit polyclonal antibody to MEK5 (MAP2K5) from human, mouse and rat. Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human MAP2K5 / MEK5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MAP2K5 / MEK5 antibody was raised against recombinant Human Dual specificity mitogen-activated protein kinase kinase 5 protein (1-190AA)
Specificity
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MAP2K5 / MEK5
Q13163 NM_145160 NP_660143.1

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MAP2K5 / MEK5 antibody Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Images

Immunohistochemistry - Paraffin

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.

Immunofluorescence

Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry - Paraffin

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.

Immunofluorescence

Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry - Paraffin

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.

Immunofluorescence

Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry - Paraffin

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.

Immunofluorescence

Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using MAP2K5 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Requested From: United States
Date Requested: 12/10/2018
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