Human MAP1LC3A / LC3A

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminus of LC3A (NP_115903.1; NP_852610.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Pig, Opossum, Guinea pig, Salmon, Zebrafish (100%); Ferret, Dog, Cat, Lizard, Catfish, Stickleback, Medaka, Pufferfish (93%); Chicken, Pike (86%).

aa2-15

Recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy. LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane. Recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

• Immunofluorescence
• Western blot (1:100 - 1:250)

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9H492 NCBI: NM_032514 NP_115903.1