Target
Human MAP1LC3A / LC3A
Synonyms
MAP1LC3A | ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Immunogen
Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminus of LC3A (NP_115903.1; NP_852610.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Pig, Opossum, Guinea pig, Salmon, Zebrafish (100%); Ferret, Dog, Cat, Lizard, Catfish, Stickleback, Medaka, Pufferfish (93%); Chicken, Pike (86%).
Specificity
Recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy. LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane. Recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.