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Antibodies > MAD1L1 / MAD1 > LS-C357425
 
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Catalog Number Size Price
LS-C357425-10 10 µg $318 
LS-C357425-100 100 µg (0.5 mg/ml) $470 
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - MAD1 antibody IHC-paraffin: Human Placenta Tissue.
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - Western blot analysis of MAD1 using anti-MAD1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. Lane 5: human HL-60 whole cell lysates. Lane 6: human THP-1 whole cell lysates. Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.
MAD1L1 / MAD1 Antibody - Western blot analysis of MAD1 using anti-MAD1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.
MAD1L1 / MAD1 Antibody - MAD1 antibody Western blot. All lanes: Anti MAD1 at 0.5 ug/ml. WB: Recombinant Human MAD1 Protein 0.5ng. Predicted band size: 50 kD. Observed band size: 50 kD.
MAD1L1 / MAD1 Antibody - MAD1 antibody Western blot. All lanes: Anti MAD1 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: JURKAT Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: 293T Whole Cell Lysate at 40 ug. Lane 5: SHG Whole Cell Lysate at 40 ug. Lane 6: 22RV1 Whole Cell Lysate at 40 ug. Lane 7: PANC Whole Cell Lysate at 40 ug. Predicted band size: 83 kD. Observed band size: 83 kD.
MAD1L1 / MAD1 Antibody - Flow Cytometry analysis of A431 cells using anti-MAD1 antibody. Overlay histogram showing A431 cells stained with anti-MAD1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAD1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - MAD1 antibody IHC-paraffin: Human Placenta Tissue.
MAD1L1 / MAD1 Antibody - IHC analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-MAD1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAD1L1 / MAD1 Antibody - Western blot analysis of MAD1 using anti-MAD1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. Lane 5: human HL-60 whole cell lysates. Lane 6: human THP-1 whole cell lysates. Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.
MAD1L1 / MAD1 Antibody - Western blot analysis of MAD1 using anti-MAD1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.
MAD1L1 / MAD1 Antibody - MAD1 antibody Western blot. All lanes: Anti MAD1 at 0.5 ug/ml. WB: Recombinant Human MAD1 Protein 0.5ng. Predicted band size: 50 kD. Observed band size: 50 kD.
MAD1L1 / MAD1 Antibody - MAD1 antibody Western blot. All lanes: Anti MAD1 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: JURKAT Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: 293T Whole Cell Lysate at 40 ug. Lane 5: SHG Whole Cell Lysate at 40 ug. Lane 6: 22RV1 Whole Cell Lysate at 40 ug. Lane 7: PANC Whole Cell Lysate at 40 ug. Predicted band size: 83 kD. Observed band size: 83 kD.
MAD1L1 / MAD1 Antibody - Flow Cytometry analysis of A431 cells using anti-MAD1 antibody. Overlay histogram showing A431 cells stained with anti-MAD1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAD1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human MAD1L1 / MAD1 Antibody (aa362‑632, IHC, WB) LS‑C357425

Polyclonal Rabbit anti‑Human MAD1L1 / MAD1 Antibody (aa362‑632, IHC, WB) LS‑C357425

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Note: This antibody replaces LS-C388798
Antibody:
MAD1L1 / MAD1 Rabbit anti-Human Polyclonal (aa362-632) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357425-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MAD1L1 / MAD1 Rabbit anti-Human Polyclonal (aa362-632) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MAD1 antibody LS-C357425 is an unconjugated rabbit polyclonal antibody to MAD1 (MAD1L1) (aa362-632) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human MAD1L1 / MAD1
Synonyms
MAD1L1 | HMAD1 | MAD1 | MAD1-like protein 1 | Tax-binding protein 181 | TP53I9 | PIG9 | HSMAD1 | Tax1-binding protein txbp181 | TXBP181
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1.
Epitope
aa362-632
Specificity
Expressed weakly at G0/G1 and highly at late S and G2/M phase.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAD1L1 / MAD1
Q9Y6D9 NM_003550 NP_003541.2

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Date Requested: 4/30/2024
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