Macrophage

Mouse

Rat (tested or 100% immunogen sequence identity)

Monoclonal

Ki-M2R

Unconjugated

Affinity purified

Unmodified

Peritoneal macrophages

Ki-M2R recognizes a surface antigen of 45kD molecular weight as a single band under reducing conditions. The epitope has not been further characterized Antigen distribution: Isolated cells: In the bone marrow a minor number of macrophages reveal a strong reactivity whereas granulocytes, erythropoetic cells and megacaryocytes are negative. Immature accessory cells such as dendritic or interdigitating reticulum cells are also negative. Monocytes show a positive reaction when exposed to TPA or foreign material. Tissue sections: Ki-M2R positive cells have been recognised as active phagocytes in serous cavities (peritoneal and pleural macrophages). Liver (Kupffer cells), spleen (macrophages of the red pulp), lymph nodes (macrophages of the pulp and sinus, tingible body macrophages), lung (approx. 50% of alveolar macrophages) and connective tissue (histiocytes) are positively stained. Plasma cells (excluding stimulated monocytes) as well as sinus lining cells are negative. Specificity: Rat: mature macrophages. Other species: not tested

• IHC
• IHC - Paraffin (1:400)

Lyophilized from PBS, pH 7.2, 5 mg/ml BSA, 0.09% Sodium Azide

Reconstitute with 0.5ml distilled H2O.

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

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