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Catalog Number Size Price
LS-C662950-10 10 µg $318 
LS-C662950-100 100 µg $470 
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody. LGALS3BP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-LGALS3BP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - Western blot analysis of LGALS3BP using anti-LGALS3BP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGALS3BP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LGALS3BP at approximately 75KD. The expected band size for LGALS3BP is at 65KD.
MAC-2-BP / LGALS3BP Antibody - Western blot analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody. LGALS3BP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-LGALS3BP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - IHC analysis of LGALS3BP using anti-LGALS3BP antibody
MAC-2-BP / LGALS3BP Antibody - Western blot analysis of LGALS3BP using anti-LGALS3BP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGALS3BP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LGALS3BP at approximately 75KD. The expected band size for LGALS3BP is at 65KD.
MAC-2-BP / LGALS3BP Antibody - Western blot analysis of LGALS3BP using anti-LGALS3BP antibody
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Polyclonal Rabbit anti‑Human MAC‑2‑BP / LGALS3BP Antibody (IHC, WB) LS‑C662950

Polyclonal Rabbit anti‑Human MAC‑2‑BP / LGALS3BP Antibody (IHC, WB) LS‑C662950

Antibody:
MAC-2-BP / LGALS3BP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662950-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAC-2-BP / LGALS3BP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LGALS3BP antibody LS-C662950 is an unconjugated rabbit polyclonal antibody to human LGALS3BP (MAC-2-BP). Validated for IHC and WB.
Target
Human MAC-2-BP / LGALS3BP
Synonyms
LGALS3BP | 90K | Galectin-3-binding protein | Mac-2 BP | Mac-2-binding protein | MAC2BP | L3 antigen | Serum protein 90K | TANGO10B | BTBD17B | M2BP | MAC-2-BP | Tumor-associated antigen 90K
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence of human LGALS3BP (HEALFQKKTLQALEFHTVPFQLLARYKGLNLTEDTYKPR).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAC-2-BP / LGALS3BP
Q08380 NM_005567 NP_005558.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024