Target
Human M-phase phosphoprotein Epitope (LTPLK or FTPLQ)
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Mitotic human HeLa cell cytosolic lysate.
Specificity
Recognizes a phosphorylated epitope (S/T) P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type. Species cross-reactivity: animals and plants.
Applications
- ICC
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
- ELISA
Usage
Suitable for use in Western Blot, Immunocytochemistry, ELISA and Immunoprecipitation. Immunoprecipitation: Reported by an independent laboratory. Positive control: Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 40% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee