Photinus pyralis Luciferase
(tested or 100% immunogen sequence identity)
Delipidated and defibrinated
- Western blot (1:2000 - 1:10000)
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase [Firefly]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
This product has been assayed against 1.0 ug of Luciferase [Firefly] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20000 to 1:100000 of the reconstitution concentration is suggested for this product.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
2 ml deionized water
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.