Human LTA / TNF Beta

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG,k Recombinant Monoclonal

3F12.2D3

Unconjugated

Protein A purified

Unmodified

3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTa expressed in E. coli - the hybridoma clone was selected for its ability to bind LTa3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.

Lymphotoxin alpha

3F12 binds to human LT alpha 3 and LT alpha beta (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LT alpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LT alpha 3), or is expressed on the cell surface in complex with LT beta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LT alpha 3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.

• Flow Cytometry
• ELISA
• Neutralization
• Surface Plasmon Resonance

Applications should be user optimized.

PBS, 0.02% ProClin™ 300.

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01374 NCBI: NM_000595 NP_000586.2