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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LMNB1 / Lamin B1 Antibody (aa526-537) IHC-plus™

Note: This antibody replaces LS-C186535
Lamin B1 antibody LS-B11184 is an unconjugated goat polyclonal antibody to Lamin B1 (LMNB1) from human, bovine, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11184-50
50 µg (0.5 mg/ml)
$425
Description
Lamin B1 antibody LS-B11184 is an unconjugated goat polyclonal antibody to Lamin B1 (LMNB1) from human, bovine, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human LMNB1 / Lamin B1
Host
Goat
Reactivity
Human, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-DVKVILKNSQGE from an internal region (near C-terminus) of human LMNB1 / Lamin B1 (NP_005564.1; NP_001185486.1). Percent identity by BLAST analysis: Hamster, Panda, Bovine, Dog, Cat, Horse, Rabbit, Pig, Xenopus (100%); Galago, Rat, Guinea pig, Zebra finch, Chicken (92%); Lizard (83%).
Blocking Peptide
LS-E27951 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11184. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa526-537
Specificity
Human LMNB1 / Lamin B1. This antibody is expected to recognize both reported isoforms (NP_005564.1; NP_001185486.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 70kD band observed in nuclear lysates of cell lines HeLa and Jurkat (calculated MW of 66.4kD according to NP_005564.1). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About LMNB1 / Lamin B1
P20700 NM_005573 NP_005564.1

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Images

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 2/16/2019
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