LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
LMNB1 / Lamin B1 Antibody (aa526‑537) IHC‑plus™ LS‑B11184
Note: This antibody replaces LS-C186535
Lamin B1 antibody LS-B11184 is an unconjugated goat polyclonal antibody to Lamin B1 (LMNB1) from human, bovine, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11184-50
50 µg (0.5 mg/ml)
$425

Popular LMNB1 / Lamin B1 Products

Antibody
Species: Chicken, Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Anti-LMNB1 / Lamin B1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6062 concentration 3 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-LMNB1 / Lamin B1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10762 concentration 7.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot of LMNB1 / Lamin B1 antibody.
Species: Human, Pig
Applications: IHC, Western blot

Product Description

Lamin B1 antibody LS-B11184 is an unconjugated goat polyclonal antibody to Lamin B1 (LMNB1) from human, bovine, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About LMNB1 / Lamin B1
In animal cells, there are A- and B-type lamins, which differ in their length and isoelectric point (pI). Human cells have three differentially regulated genes. B-type lamins are present in every cell. B type lamins, B1 and B2, are expressed from the LMNB1 and LMNB2 genes on 5q23 and 19q13, respectively. A-type lamins are expressed only following gastrulation. Lamin A and C are the most common A-type lamins and are splice variants of the LMNA gene found at 1q21. P20700 NM_005573 NP_005564.1

LMNB1 Antibody, ADLD Antibody, Lamin B1 Antibody, Lamin-B1 Antibody, LMN Antibody, LMNB Antibody, LMN2 Antibody


Specifications

Target
Human LMNB1 / Lamin B1
Host
Goat
Reactivity
Human, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-DVKVILKNSQGE from an internal region (near C-terminus) of human LMNB1 / Lamin B1 (NP_005564.1; NP_001185486.1). Percent identity by BLAST analysis: Hamster, Panda, Bovine, Dog, Cat, Horse, Rabbit, Pig, Xenopus (100%); Galago, Rat, Guinea pig, Zebra finch, Chicken (92%); Lizard (83%).
Blocking Peptide
LS-E27951 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11184. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa526-537
Specificity
Human LMNB1 / Lamin B1. This antibody is expected to recognize both reported isoforms (NP_005564.1; NP_001185486.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 70kD band observed in nuclear lysates of cell lines HeLa and Jurkat (calculated MW of 66.4kD according to NP_005564.1). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.
Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11184 concentration 5 ug/ml.

Western blot

LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 9/20/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy