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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LMNB1 / Lamin B1 Antibody (aa124‑137) LS‑C186536
Lamin B1 antibody LS-C186536 is an unconjugated goat polyclonal antibody to human Lamin B1 (LMNB1). Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C186536-100
100 µg (0.5 mg/ml)
$405
Description
Lamin B1 antibody LS-C186536 is an unconjugated goat polyclonal antibody to human Lamin B1 (LMNB1). Validated for Peptide-ELISA and WB.
Target
Human LMNB1 / Lamin B1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Dog, Hamster, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
Synthetic peptide C-KESDLNGAQIKLRE from an internal region of human LMNB1 / Lamin B1 (NP_005564.1). Percent identity by BLAST analysis: Hamster, Panda, Dog, Horse (93%).
Blocking Peptide
LS-E27950 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186536. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa124-137
Specificity
Human LMNB1 / Lamin B1. This antibody is expected to recognize isoform 1 (NP_005564.1) only.
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 65kD band observed in lysates of cell line Jurkat (calculated MW of 66.4kD according to NP_005564.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About LMNB1 / Lamin B1
P20700 NM_005573 NP_005564.1

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Images

Western blot

LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LMNB1 antibody (0.2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 11/19/2018
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